682 research outputs found
Seven challenges for model-driven data collection in experimental and observational studies
Infectious disease models are both concise statements of hypotheses and powerful techniques for creating tools from hypotheses and theories. As such, they have tremendous potential for guiding data collection in experimental and observational studies, leading to more efficient testing of hypotheses and more robust study designs. In numerous instances, infectious disease models have played a key role in informing data collection, including the Garki project studying malaria, the response to the 2009 pandemic of H1N1 influenza in the United Kingdom and studies of T-cell immunodynamics in mammals. However, such synergies remain the exception rather than the rule; and a close marriage of dynamic modeling and empirical data collection is far from the norm in infectious disease research. Overcoming the challenges to using models to inform data collection has the potential to accelerate innovation and to improve practice in how we deal with infectious disease threats
Evaluation and construction of diagnostic criteria for inclusion body myositis
OBJECTIVE:
To use patient data to evaluate and construct diagnostic criteria for inclusion body myositis (IBM), a progressive disease of skeletal muscle.
METHODS:
The literature was reviewed to identify all previously proposed IBM diagnostic criteria. These criteria were applied through medical records review to 200 patients diagnosed as having IBM and 171 patients diagnosed as having a muscle disease other than IBM by neuromuscular specialists at 2 institutions, and to a validating set of 66 additional patients with IBM from 2 other institutions. Machine learning techniques were used for unbiased construction of diagnostic criteria.
RESULTS:
Twenty-four previously proposed IBM diagnostic categories were identified. Twelve categories all performed with high (≥97%) specificity but varied substantially in their sensitivities (11%-84%). The best performing category was European Neuromuscular Centre 2013 probable (sensitivity of 84%). Specialized pathologic features and newly introduced strength criteria (comparative knee extension/hip flexion strength) performed poorly. Unbiased data-directed analysis of 20 features in 371 patients resulted in construction of higher-performing data-derived diagnostic criteria (90% sensitivity and 96% specificity).
CONCLUSIONS:
Published expert consensus-derived IBM diagnostic categories have uniformly high specificity but wide-ranging sensitivities. High-performing IBM diagnostic category criteria can be developed directly from principled unbiased analysis of patient data.
CLASSIFICATION OF EVIDENCE:
This study provides Class II evidence that published expert consensus-derived IBM diagnostic categories accurately distinguish IBM from other muscle disease with high specificity but wide-ranging sensitivities
The role of caretakers in disease dynamics
One of the key challenges in modeling the dynamics of contagion phenomena is
to understand how the structure of social interactions shapes the time course
of a disease. Complex network theory has provided significant advances in this
context. However, awareness of an epidemic in a population typically yields
behavioral changes that correspond to changes in the network structure on which
the disease evolves. This feedback mechanism has not been investigated in
depth. For example, one would intuitively expect susceptible individuals to
avoid other infecteds. However, doctors treating patients or parents tending
sick children may also increase the amount of contact made with an infecteds,
in an effort to speed up recovery but also exposing themselves to higher risks
of infection. We study the role of these caretaker links in an adaptive network
models where individuals react to a disease by increasing or decreasing the
amount of contact they make with infected individuals. We find that pure
avoidance, with only few caretaker links, is the best strategy for curtailing
an SIS disease in networks that possess a large topological variability. In
more homogeneous networks, disease prevalence is decreased for low
concentrations of caretakers whereas a high prevalence emerges if caretaker
concentration passes a well defined critical value.Comment: 8 pages, 9 figure
Steady-State Dynamics of the Forest Fire Model on Complex Networks
Many sociological networks, as well as biological and technological ones, can
be represented in terms of complex networks with a heterogeneous connectivity
pattern. Dynamical processes taking place on top of them can be very much
influenced by this topological fact. In this paper we consider a paradigmatic
model of non-equilibrium dynamics, namely the forest fire model, whose
relevance lies in its capacity to represent several epidemic processes in a
general parametrization. We study the behavior of this model in complex
networks by developing the corresponding heterogeneous mean-field theory and
solving it in its steady state. We provide exact and approximate expressions
for homogeneous networks and several instances of heterogeneous networks. A
comparison of our analytical results with extensive numerical simulations
allows to draw the region of the parameter space in which heterogeneous
mean-field theory provides an accurate description of the dynamics, and
enlights the limits of validity of the mean-field theory in situations where
dynamical correlations become important.Comment: 13 pages, 9 figure
Tibiofemoral Contact Forces in the Anterior Cruciate Ligament-Reconstructed Knee.
PURPOSE: To investigate differences in ACL reconstructed (ACLR) and healthy individuals in terms of the magnitude of the tibiofemoral contact forces, as well as the relative muscle and external load contributions to those contact forces, during walking, running and sidestepping gait tasks. METHODS: A computational electromyography-driven neuromusculoskeletal model was used to estimate the muscle and tibiofemoral contact forces in those with combined semitendinosus and gracilis tendon autograft ACLR (n=104, 29.7±6.5 years, 78.1±14.4 kg) and healthy controls (n=60, 27.5±5.4 years, 67.8±14.0 kg) during walking (1.4±0.2 ms), running (4.5±0.5 ms) and sidestepping (3.7±0.6 ms). Within the computational model, the semitendinosus of ACLR participants was adjusted to account for literature reported strength deficits and morphological changes subsequent to autograft harvesting. RESULTS: ACLRs had smaller maximum total and medial tibiofemoral contact forces (~80% of control values, scaled to bodyweight) during the different gait tasks. Compared to controls, ACLRs were found to have a smaller maximum knee flexion moment, which explained the smaller tibiofemoral contact forces. Similarly, compared to controls, ACLRs had both a smaller maximum knee flexion angle and knee flexion excursion during running and sidestepping, which may have concentrated the articular contact forces to smaller areas within the tibiofemoral joint. Mean relative muscle and external load contributions to the tibiofemoral contact forces were not significantly different between ACLRs and controls. CONCLUSION: ACLRs had lower bodyweight-scaled tibiofemoral contact forces during walking, running and sidestepping, likely due to lower knee flexion moments and straighter knee during the different gait tasks. The relative contributions of muscles and external loads to the contact forces were equivalent between groups
Topology and correlations in structured scale-free networks
We study a recently introduced class of scale-free networks showing a high
clustering coefficient and non-trivial connectivity correlations. We find that
the connectivity probability distribution strongly depends on the fine details
of the model. We solve exactly the case of low average connectivity, providing
also exact expressions for the clustering and degree correlation functions. The
model also exhibits a lack of small world properties in the whole parameters
range. We discuss the physical properties of these networks in the light of the
present detailed analysis.Comment: 10 pages, 9 figure
The spread of epidemic disease on networks
The study of social networks, and in particular the spread of disease on
networks, has attracted considerable recent attention in the physics community.
In this paper, we show that a large class of standard epidemiological models,
the so-called susceptible/infective/removed (SIR) models can be solved exactly
on a wide variety of networks. In addition to the standard but unrealistic case
of fixed infectiveness time and fixed and uncorrelated probability of
transmission between all pairs of individuals, we solve cases in which times
and probabilities are non-uniform and correlated. We also consider one simple
case of an epidemic in a structured population, that of a sexually transmitted
disease in a population divided into men and women. We confirm the correctness
of our exact solutions with numerical simulations of SIR epidemics on networks.Comment: 12 pages, 3 figure
Signatures of small-world and scale-free properties in large computer programs
A large computer program is typically divided into many hundreds or even
thousands of smaller units, whose logical connections define a network in a
natural way. This network reflects the internal structure of the program, and
defines the ``information flow'' within the program. We show that, (1) due to
its growth in time this network displays a scale-free feature in that the
probability of the number of links at a node obeys a power-law distribution,
and (2) as a result of performance optimization of the program the network has
a small-world structure. We believe that these features are generic for large
computer programs. Our work extends the previous studies on growing networks,
which have mostly been for physical networks, to the domain of computer
software.Comment: 4 pages, 1 figure, to appear in Phys. Rev.
Glutathione and Gts1p drive beneficial variability in the cadmium resistances of individual yeast cells
Phenotypic heterogeneity among individual cells within isogenic populations is widely documented, but its consequences are not well understood. Here, cell-to-cell variation in the stress resistance of Saccharomyces cerevisiae, particularly to cadmium, was revealed to depend on the antioxidant glutathione. Heterogeneity was decreased strikingly in gsh1 mutants. Furthermore, cells sorted according to differing reduced-glutathione (GSH) contents exhibited differing stress resistances. The vacuolar GSH-conjugate pathway of detoxification was implicated in heterogeneous Cd resistance. Metabolic oscillations (ultradian rhythms) in yeast are known to modulate single-cell redox and GSH status. Gts1p stabilizes these oscillations and was found to be required for heterogeneous Cd and hydrogen-peroxide resistance, through the same pathway as Gsh1p. Expression of GTS1 from a constitutive tet-regulated promoter suppressed oscillations and heterogeneity in GSH content, and resulted in decreased variation in stress resistance. This enabled manipulation of the degree of gene expression noise in cultures. It was shown that cells expressing Gts1p heterogeneously had a competitive advantage over more-homogeneous cell populations (with the same mean Gts1p expression), under continuous and fluctuating stress conditions. The results establish a novel molecular mechanism for single-cell heterogeneity, and demonstrate experimentally fitness advantages that depend on deterministic variation in gene expression within cell populations
Model-consistent estimation of the basic reproduction number from the incidence of an emerging infection
We investigate the merit of deriving an estimate of the basic reproduction number \documentclass[12pt]{minimal}
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, and we quantify the discrepancies that arise
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